Advancing next generation therapeutics requires reliable characterization of biotherapeutic binding and biomolecular stability. Characterizing biological macromolecules is important for understanding their functions and relationships, which empowers the development of new therapies and treatments. In the case of biotherapeutic molecules, changes in structure could unexpectedly reduce or enhance drug product quality and safety.

Microcalorimetry is a highly sensitive technique for measuring enthalpy and heat capacity changes during reactions. Two microcalorimetry techniques, differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC), are the standard methods for characterizing macromolecules’ intermolecular binding and structural stability. DSC reveals strengths and weaknesses in higher order structure and defines the behavior of individual domains and their interactions. Information from Nano DSC experiments enable scientists to make the best formulations, as well as monitor changes in their product over time.

This eBook contains five real experiments that will teach you how to:

• Assess biopharmaceuticals qualitatively and quantitatively – including differentiation between conjugate types and drug-to-antibody ratios
• Determine ideal formulation conditions and identify changes during drug manufacturing or storage
• Gain a clear picture of the driving forces behind a binding interaction
• Identify key considerations when selecting a specific model for analyzing thermograms
• Get a better understanding on how proteins interact with each other and how protonation can affect binding affinity

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