ITC has recently received increased attention from the field of material science, which necessitates the use of non-aqueous solvent systems. Herein, we elaborate on the development of a representative binding assay that measures the interactions of Ca2+ with a calcium ionophore in a methanol solvent system.
DetailsThe interaction between the antigen binding site and an antigen is extremely specific, which has made antibodies very highly desirable reagents when developing a wide variety of antigen detection assays.
DetailsIsothermal titration calorimetry is a technique where heat is measured under constant pressure. This heat can be translated into enthalpy based on the First Law of Thermodynamics. Although the focus of the technique has been rooted in measuring biophysical phenomena in labs that specialized in calorimetry, it has seen growth as a general technique to quantitatively describe systems from proteins to nanomaterials.
DetailsNano Differential Scanning Calorimetry (Nano DSC) and Isothermal Titration Calorimetry (ITC) are powerful label-free and immobilization free analytic tools for thermodynamic measurements of molecular binding, biomolecular thermostability, and enzyme kinetic reactions.
DetailsNano Differential Scanning Calorimetry (Nano DSC) and Isothermal Titration Calorimetry (ITC) are powerful label-free and immobilization free analytic tools for thermodynamic measurements of molecular binding, biomolecular thermostability, and enzyme kinetic reactions.
DetailsDo your patients worry about their replacement hip breaking when they run? Or how long their prosthetic leg will last before needing a new one? Are your regulatory submissions requiring increasingly more lifetime testing?
DetailsMechanical testing techniques are commonly used to quantify the compression characteristics of such devices. The goal of these tests is to determine a relationship between the compressive displacement and the resulting force on the devices. A precise and accurate assessment of force against displacement can be performed by using the ElectroForce® 3200 Series III test instrument.
DetailsPolymer leaflet heart valves can fail in long-term use due to tearing and calcification caused by high dynamic tensile and bending stresses on the material and oxidative reactions with blood. It was postulated that synthetic valve leaflets that mimic the natural valve leaflet structure fabricated from fiber-reinforced composite material will minimize leaflet stresses and decrease tears and perforations.
DetailsTA Instruments’ 3300 and 3510 load frames can be used to characterize the mechanical strength and durability of breast implants. These insights are crucial for both the design optimization and final validation that is required for regulation.
DetailsHydrogels are used for a range of applications. In this study, we characterized two different types of topical wound dressing hydrogels, one specialized for radiation dermatitis, and another for low exudating wounds using dynamic mechanical analysis (DMA) techniques.
DetailsA rheometer is a powerful instrument that can be used to monitor the gel formation and also to measure the gel strength. Two case studies are discussed regarding the use of a TA Instruments’ rotational rheometer to analyze the properties of different types of hydrogels.
DetailsThis study had a threefold purpose: 1) study the creep response of bone to repetitive phsyiologically-relevant loading 2) to perform these types of tests on a small scale; and, 3) to illustrate and identify age-related effects on the mechanical properties of bone.
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